ANIMATIC - APRIL 11th

Animatic - Based on script version 18. April 11, 2010.

Visual Style

I've been looking around at demo reels, and the one by Random 42 based in the UK caught my attention.

The best way to describe it... they use a cold but soft light. Kind of looks sterile, laboratory-like. Very reminiscent of the cold northern BC skies... Or the dreary UK winters?

The image above is a screen capture from their showreel at www.random42.com.

ANIMATIC

Animatic = somewhat animated or slideshow preview of storyboard.

NOTE: The narration is from the OLD script, but nevertheless, it's what was handed in for my storyboard course. Hopefully it'll give a good idea of the feel of the film so far...

Animatic for course using old script.

SEGMENT 6 SCENE 1 - CONCLUSION

In metastatic bone cancer,

pain does not simply

come from a single source.

Various stresses, be they

mechanical or chemical,

layer upon each other

to create a severe

and unmanageable neuropathy.

Owing to the plasticity

of neurons, pathological

changes soon extend from here

to the central nervous system.

And, to think that

all this starts

with a few cells.

Approximate run time: 31 seconds

UPDATE 09/12/12

So here's the final leg of the film. This segment was animated for the old script. So it WILL BE CHANGED as well. I need to go back to the drafting table and try and tie in the CNS and spinal cord a little more...

But what I have here at the moment, is an attempt to bridge the intro and the end. We start off will a few cells, so I wanted to end on that note. It felt a little... unresolved when I had originally ended with a scene of utter chaos - with nociceptive factors everywhere and things firing like crazy...

Segment 6 Scene 1

SEGMENT 5 SCENE 2 - ACIDOSIS

In the low pH,

acid-sensing ion channels

cause nerves

to readily depolarize.

Constant activation of these channels

lead to gradient imbalances,

priming neurons for spontaneous firing.

With the hypersensitization

of these nociceptors,

chronic pain settles in.

Approximate run time: 22 seconds

This section also needs some expansion.

UPDATE 09/12/11

Currently, I have the acid ions binding to the ASIC receptors (not yet labelled - will be labelled immediately under each structure). Then I have the neuron changing colours to represent the gradient imbalance. This is where I would love to get some feedback. Is it effective? Or not?

The reason I did not show ions passing through the bilayer is that I had already established/introduced H+, which for the purpose of clarity, has been blown up by a lot. To now introduce other ions at that scale would be difficult because they wouldn't be able to flow through all that easily (they would fit through channel). And now, suppose that we make them small dots of light flowing through the channel, then we have a discontinuity between ions and H+.

Also... How exactly does H+ bind or activate ASICs...?

Segment 5 Scene 2

SEGMENT 5 SCENE 1 - OSTEOCLASTS

Like macrophages,

osteoclasts release acids.

They do so in order

to dissolve the

minerals in the bone.

Therefore, osteoclast hyperactivity

aside from resorbing bone,

creates a highly

acidic environment as well.

Approximate run-time: 20 seconds

This sections needs to be expand.

Segment 5 Scene 1

SEGMENT 4 SCENE 2 - INFLAMMATORY NOCICEPTION

And caught in the crossfire

is a sea of nerve endings,

whose surface is littered

by an abundance of receptors

and channels.

Their overstimulation

leads to the inflammatory

pain associated with so

many pathologies.

But, in our case, we have

some cells further

complicating the situation.

Approximate run time: 22 seconds

UPDATE 09/12/09

In the video, I only have protons and ATPs binding so far. But as a continuation from the previous video, I plan on the series of factors introduced previously, floating downwards to activate their respective receptors. The main point I'm trying to get across is that all the factors released in the previous scene will lend itself to the activation of the neurons.

Segment 4 Scene 2